Collaboration Article: Glucose Concentration in Regulating Induced Pluripotent Stem Cells Differentiation Toward Insulin-Producing Cells

We are very happy to annouce the publication of a study led by Dr. Hanne Scholz focusing on the impact of glucose concentration in regulating the differentiation of induced pluripotent stem cells toward insulin-producing cells. We previously showed that chronically elevated exogenous glucose elicits antipodal effects on the proteome signature of differentiating human iPSC-derived pancreatic progenitors.

Here, Chencheng focused on comparing high (20 mM) versus low (5.5 mM) glucose concentrations on IPCs differentiation in three hiPSC lines. This study shows that high glucose during differentiation leads to decreased number of NKX6.1+ cells co-expression with PDX1 due to insufficient NKX6.1 gene activation, and thus reducing further the differentiation efficiency. Also,  differentiation in high glucose medium weakened mitochondrial respiration ability. However glucose-stimulated insulin secretion was only seen in the differentiation under a high glucose condition. These cells have higher KATP channel activity and were linked to sufficient ABCC8 gene expression under a high glucose condition. This study proposes that high glucose concentration in the culture medium during IPC differentiation is necessary to generate functional islet-like cells. However, in cell lines that are less prone to induced differentiation, high glucose could worsen the situation.

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Glucose Concentration in Regulating Induced Pluripotent Stem Cells Differentiation Toward Insulin-Producing Cells

Authors: Wang C, Abadpour S, Olsen PA, Wang D, Stokowiec J, Chera S, Ghila L, Ræder H, Krauss S, Aizenshtadt A, Scholz H

Transplant International 2024 Jan 18:11900

DOI information: 10.3389/ti.2024.11900