Our recent study on iPSC-derived human pancreatic progenitors initial response to in vivo is now published:
- The main goal of this study was to demultiplex and characterize the initial response of the hiPSC-derived differentiating pancreatic progenitors to the in vivo environment, by using global proteomics and large-scale imaging techniques.
- We showed that the in vivo exposure rapidly routes a large fraction of human pancreatic progenitors toward single hormone expression.
- The overall proteome landscape of the transplanted pancreatic progenitor cells was closer to a native islet-like regulation pattern and especially the energy metabolism and redox signature.
- We identified by pathway analysis two upstream regulators, HNF1A and HNF4A predicted to be responsible for the in vivo islet promoting response of the transplanted cells and experimentally confirmed their role in confining human pancreatic progenitors to single hormone expression.
Continue your reading here:
Authors: Legøy TA, Mathisen AF, Salim Z, Vethe H, Bjørlykke Y, Abadpour S, Paulo JA, Scholz H, Raeder H, Ghila L, Chera S. Frontiers in Cell and Developmental Biology 2020 25 February 2020
DOI information: https://doi.org/10.3389/fcell.2020.00109