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Tag: Pancreatic endocrine progenitors
Research Paper: Defective HNF1A hinders GLI3 processing favoring duodenal versus pancreatic fate, thus leading to intestinal elongation in vivo
We are very happy to share our latest work on how HNF1A’s involvement in GLI3 processing affects pancreatic and intestinal development, using a combination of in vivo work (multiple conditional mouse models) and human iPSC models is now ONLINE ahead of print in Genes and Development
Research Paper: The age-dependent regulation of pancreatic islet landscape is fueled by a HNF1a-immune signaling loop
Here, Andreas built and analysed several timelines along the first year of life of different mouse models to understand how pancreatic endocrine islets are maturing and then age. By combining transgenic systems, global transcriptomics, pathway analysis, cell biology and physiology,…
Research Paper: The GLI code controls HNF1A levels during foregut differentiation
During pancreas development the HH-signaling modulations plays an essential role, its inactivation being absolutely required for pancreas organogenesis, even slight ectopic activation compromising this process. Moreover, the pathway also plays a role in adult pancreas regeneration, its inactivation promoting regenerative…
Research Paper: Global proteomics reveals insulin abundance as a marker of human islet homeostasis alterations
Here, we designed a pilot study to comprehensively identify the diversity of global proteome signatures and the deviation from normal homeostasis in randomly selected human-isolated islet samples. By using Tandem Mass Tag 16-plex proteomics, we focused on the recurrently observed…
Book Chapter: Targeted Gene Silencing by Using GapmeRs in Differentiating Human-Induced Pluripotent Stem Cells (hiPSC) Toward Pancreatic Progenitors
Induced pluripotent stem cells as a source for generating pancreatic islet endocrine cells represent a great research tool for deciphering the molecular mechanisms of lineage commitment, a layered multi-step process. Additionally, targeted gene silencing by using GapmeRs, short antisense oligonucleotides,…
Collaboration Paper: Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived β-Like Cells
For the past five years, our collaborator Helge Ræder and us showed that human induced pluripotent stem cells (hiPSC) provide an excellent tool for disease modelling. However, in vitro differentiation is highly sensitive to culture conditions. By re-creating a spatial…