Here, Andreas built and analysed several timelines along the first year of life of different mouse models to understand how pancreatic endocrine islets are maturing and then age. By combining transgenic systems, global transcriptomics, pathway analysis, cell biology and physiology,…
During pancreas development the HH-signaling modulations plays an essential role, its inactivation being absolutely required for pancreas organogenesis, even slight ectopic activation compromising this process. Moreover, the pathway also plays a role in adult pancreas regeneration, its inactivation promoting regenerative…
Here, we designed a pilot study to comprehensively identify the diversity of global proteome signatures and the deviation from normal homeostasis in randomly selected human-isolated islet samples. By using Tandem Mass Tag 16-plex proteomics, we focused on the recurrently observed…
Induced pluripotent stem cells as a source for generating pancreatic islet endocrine cells represent a great research tool for deciphering the molecular mechanisms of lineage commitment, a layered multi-step process. Additionally, targeted gene silencing by using GapmeRs, short antisense oligonucleotides,…
For the past five years, our collaborator Helge Ræder and us showed that human induced pluripotent stem cells (hiPSC) provide an excellent tool for disease modelling. However, in vitro differentiation is highly sensitive to culture conditions. By re-creating a spatial…
Our latest work on iPSC-derived human pancreatic progenitors differentiation is now out for Early View: The main goal of this study was to assess the effects of elevated glucose concentrations exposure during the last stages of guided in vitro differentiation…
Pancreatic islet endocrine cells generated from patient-derived induced pluripotent stem cells represent a great strategy for both disease modeling and regenerative medicine. Nevertheless, these cells inherently miss the effects of the intricate network of systemic signals characterizing the living organisms.…