Tag: Lucas Unger

Journal Club: CRISPR screening uncovers a central requirement for HHEX in pancreatic lineage commitment and plasticity restriction

by Lucas Unger Yang et al. demonstrate that HHEX is a lineage-restricting transcription factor essential for pancreas specification from foregut progenitors. Through CRISPR-Cas9 loss-of-function screening combined with hiPSC differentiation and single-cell multiomics, the study reveals that deletion of HHEX diverts…

Journal Club: Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells

by Lucas Unger The differentiation of human pluripotent stem cells into insulin-producing beta cells holds great potential for both transplantation-based treatment of insulin-dependent diabetes and diabetes research. In this study, a comprehensive analysis of publicly available single-cell RNA sequencing data…